全文获取类型
收费全文 | 2084篇 |
免费 | 110篇 |
国内免费 | 230篇 |
专业分类
林业 | 214篇 |
农学 | 119篇 |
基础科学 | 123篇 |
396篇 | |
综合类 | 711篇 |
农作物 | 97篇 |
水产渔业 | 189篇 |
畜牧兽医 | 310篇 |
园艺 | 153篇 |
植物保护 | 112篇 |
出版年
2024年 | 9篇 |
2023年 | 53篇 |
2022年 | 60篇 |
2021年 | 94篇 |
2020年 | 82篇 |
2019年 | 86篇 |
2018年 | 66篇 |
2017年 | 85篇 |
2016年 | 98篇 |
2015年 | 87篇 |
2014年 | 103篇 |
2013年 | 120篇 |
2012年 | 151篇 |
2011年 | 174篇 |
2010年 | 104篇 |
2009年 | 120篇 |
2008年 | 97篇 |
2007年 | 116篇 |
2006年 | 109篇 |
2005年 | 83篇 |
2004年 | 59篇 |
2003年 | 56篇 |
2002年 | 53篇 |
2001年 | 45篇 |
2000年 | 36篇 |
1999年 | 34篇 |
1998年 | 24篇 |
1997年 | 30篇 |
1996年 | 19篇 |
1995年 | 30篇 |
1994年 | 23篇 |
1993年 | 27篇 |
1992年 | 17篇 |
1991年 | 27篇 |
1990年 | 15篇 |
1989年 | 8篇 |
1988年 | 8篇 |
1987年 | 5篇 |
1986年 | 4篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1980年 | 1篇 |
1956年 | 2篇 |
排序方式: 共有2424条查询结果,搜索用时 15 毫秒
51.
Davis EG Wilkerson MJ Rush BR 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2002,16(4):404-410
The use of flow cytometry in veterinary diagnostics is becoming a valuable clinical tool with a broad range of applications. Physical characteristics of cells can be determined by the flow cytometer laser and electronics through the measurement of changes in light scatter properties. Other components and functions of cells can be defined through the application of fluorochrome dyes that have an affinity for cellular components. Traditionally, common clinical applications are immunophenotyping of cells of the hematopoietic system with fluorescent-labeled antibodies raised against specific cell surface proteins. Other approaches have been used to elucidate changes in cell function and DNA content. This review is intended to provide the reader with the fundamental uses of flow cytometry. Examples of clinical applications in equine patients include immune-mediated hemolytic anemia, immune-mediated thrombocytopenia (IMT), chronic inflammatory disease, and neoplasia. 相似文献
52.
We examined exogenous and endogenous development of Eimeria procera in experimentally infected grey partridges (Perdix perdix). Our examination included data on morphology, localization, duration of schizogony and gametogony and morphology of sporulated oocysts. The endogenous stages of E. procera developed in large numbers within the epithelial cells of caecal crypts. The asexual development comprised three generations of schizonts. The first fully developed macrogametes and microgamonts were observed on Day 5 post-infection (p.i.) in histologic section. The patent period began on Day 6 p.i. and ended on Day 11 p.i. with peak production of oocysts on Days 7 and 8. Long oval oocysts of E. procera measured 25.78–28.13 μm in length and 14.06–15.24 μm in width, sporulation time ranged from 18 to 24 h at 25°C and from 36 to 48 h at 20°C. 相似文献
53.
大鼠发情周期各阶段的阴道细胞变化观察 总被引:10,自引:0,他引:10
为了比较准确的确定大鼠发情周期各个阶段,利用阴道组织涂片法,对14只6月龄雌性Wistar大鼠连续20 d进行阴道涂片,镜检,观察阴道涂片中细胞的变化规律。结果表明,在发情间期,阴道涂片以白细胞为主(84.6个±1.1个);发情前期以有核上皮细胞为主(72.4个±8.3个);在发情期,以角质化的上皮细胞为主(78个±5.4个);发情后期角质化上皮细胞、有核上皮细胞和白细胞3种细胞都有,而且比例相当。阴道涂片法能简便有效的区分大鼠发情周期的各个阶段。 相似文献
54.
运用组织学技术对36头处于发情周期的健康成年母牦牛卵巢卵泡的结构与发育状况进行了观察。结果表明,牦牛发情周期中卵巢卵泡发育的组织结构与其他牛基本相似。每对卵巢中原始卵泡数和生长卵泡数在发情前期、发情期、发情后期和发情间期之间差异不显著(P〉0.05);发情前期的囊状卵泡数与发情期、发情后期和发情间期之间差异不显著(P〉0.05),发情期和发情后期均与发情间期差异显著(P〈0.05)。闭锁生长卵泡数在4个时期之间差异不显著(P〉0.05);发情期的闭锁囊状卵泡数与其他3期之间差异极显著(P〈0.01),发情前期和发情间期均与发情后期差异显著(P〈0.05)。各级卵泡的闭锁形式各有特点。在生长卵泡、囊状卵泡及相应闭锁卵泡的卵泡膜中均见到了胶原纤维和网状纤维。 相似文献
55.
56.
Akifumi OGINO Hideki ORITO Kazuhiro SHIMADA Hiroyuki HIROOKA 《Animal Science Journal》2007,78(4):424-432
The objectives of this study were to evaluate the environmental impacts of a beef cow–calf system using a life cycle assessment (LCA) method and to investigate the effects of scenarios to reduce environmental impacts on the LCA results. The functional unit was defined as one marketed beef calf, and the processes associated with the cow–calf life cycle, such as feed production, feed transport, animal management, the biological activity of the animal and the treatment of cattle waste were included in the system boundary. The present results showed that the total contributions of one beef calf throughout its life cycle to global warming, acidification, eutrophication and energy consumption were 4550 kg of CO2 equivalents, 40.1 kg of SO2 equivalents, 7.0 kg of phosphate (PO4) equivalents and 16.1 GJ, respectively. The contribution of each process to the total environmental impact in each environmental impact category showed a similar tendency to the contribution of each process in each environmental category reported in the case of the beef fattening system as a whole. The results from this analysis showed that shortening calving intervals by 1 month reduced environmental impacts by 5.7–5.8% in all the environmental impact categories examined in the current study, and increasing the number of calves per cow also reduced environmental impacts in all the categories, although the effects were smaller. 相似文献
57.
Parul Singh Anjil Kumar Srivastava Ashok Kumar Singh 《Pesticide biochemistry and physiology》2007,89(3):216-219
In this study, the comparison of cytogenetic effects of insecticide and fungicide in different phases of cell cycle was investigated in the root tip cells of barley (Hordeum vulgare L.). The seeds of H. vulgare L. Var. Karan 16 were treated with different concentrations (0.05%, 0.1% and 0.5%) of insecticide Profenophos (PF) and fungicide Mancozeb (MZ) for 6 h after presoaking durations of 7, 17 and 27 h.The different presoaking durations were used to bring the cells in various phases of cell cycle. Negative control was run parallel in distilled water. Cytogenetic examinations of root meristems exposed to the PF and MZ showed significant inhibition of mitotic index (MI) as well as significant increase in chromosomal aberrations (CAs). These parameters were dependent on the concentrations of insecticide and fungicide. The present study shows that PF and MZ both caused more damage in S phase of cell cycle which indicates that S phase is more sensitive in comparison to other phases. 相似文献
58.
立足青海湖旅游区实际,在分析影响旅游区生命周期的诸因素基础上,提出了延长该区旅游生命周期的具体措施。 相似文献
59.
Jiménez Escobar C. Basrur P.K. Gartley C. Liptrap R.M. 《Veterinary research communications》1998,22(2):119-129
The adrenal cortex is believed to be implicated in the high incidence of abortion in the Angora goat. Stimulation testing with adrenocorticotrophic hormone (ACTH) was used to assess the adrenal cortical function in 5 Angora does from herds with a history of abortion and 5 non-Angora does. An acute test involving a single intramuscular (i.m.) injection of 0.25 mg of synthetic ACTH was given during anoestrus, at mid-oestrus, on day 90 and on day 120 of gestation. Blood samples were collected from the jugular vein at 30 min intervals for 1 h before and 5 h after injection. Cortisol concentrations rose within 30 min and returned to baseline values within 3.5 h. Cortisol production was lower (p<0.01) in the pregnant state compared to the non-pregnant state in both groups. Production of cortisol was consistently lower (p<0.05) in the Angora does compared to the non-Angora does during anoestrus and pregnancy and marginally so at mid-oestrus. A chronic stimulation test involving once daily injections of 0.5 mg of a depot form of ACTH i.m. for 7 days commencing on day 90 of pregnancy was also conducted. Cortisol concentrations rose to reach a peak on the third day of treatment in both groups. The values then declined in the Angora does despite continued ACTH treatment, while those for the non-Angora does exhibited a second peak. During and following this treatment, two non-Angora does delivered live kids (day 95, day 120). Out of 7 Angora pregnancies, one Angora doe aborted two dead fetuses at day 116. No significant difference in the cortisol response in the acute test was detected between the animals that aborted and their respective cohorts, but the two non-Angora does that aborted had significantly lower cortisol concentrations during depot ACTH administration. Progesterone and oestradiol levels did not differ between Angora and non-Angora animals during pregnancy or on the test days. The results suggest that the steroidogenic response of the adrenal cortex to ACTH stimulation is significantly less in Angora does with a history of abortion than it is in non-Angora does and support the view that the Angora goat would make a more limited adrenal cortical response to a stressful occurrence during pregnancy. 相似文献
60.